BEE Faculty - Larry Walker

Alumni

BEE Faculty

	Larry Walker, Ph.D. Professor Profile and CV	232 Riley Robb lpw1@cornell.edu Web site
Agricultural and environmental bioprocessing engineering

Research Interests

Professional CV

Education:

B.S. Physics (1974)
M.S.(1975) & Ph.D (1978) Agricultural Engineering: Michigan State University

Professional Experience

11/1/93-Present Professor (with tenure), Cornell University

2/1/85-10/31/93 Associate Professor (with tenure), Cornell University

1/2/79-1/30/85 Assistant Professor, Cornell University

1/78-12/78 Process Engineer, Tennessee Valley Authority

Sabbaticals and Study Leaves

9/1/09-Present, Visiting Scientist, Centre National De La Rescherche Scientifique, Nancy, France

1/93-6/93 Postdoctoral Fellow, Laboratoire de Biochimie et Technology des Glucides, Institut National de la Recherche Agronomique, Nantes, France

9/1/85-8/31/86 Visiting Professor, Purdue University

Research Interests:

Agricultural and environmental bioprocess engineering

Extramural Research Activities:

Director, Northeast Sun Grant Initiative
Member, American Council On Renewable Energy (ACORE), Higher Education Committee (HEC)'s Steering Committee
Member, Biomolecular Devices and Analysis Program, Cornell Nanobiotechnology Center
Co-Editor In Chief, Industrial Biotechnology
Project Director, New York State Office of Science, Technology and Academic Research (NYSTAR) Faculty Development Program Award
Member, Kavli Institute at Cornell for Nanoscale Science (KIC)

Graduate Field Experience:

Member of Agricultural Engineering Graduate Field, Cornell University (1979 - present)
Member of Chemical Engineering Graduate Field, Cornell University (2001 - present)

Professional Activities (1997 Thru Present):

Member, National Biomass Research and Development Technical Advisory Committee
Member, American Association for the Advancement of Science
Member, American Chemical Society
Member, American Society of Agricultural Engineers
Member, American Society of Microbiology
Member, Sigma Xi

Editorial Boards

Co-Editor In Chief, Industrial Biotechnology (1/2005-Present)

Member, Editorial Board of Bioresource Technology, an international journal published by Elsevier (1/87 - 6/97)

Member, Editorial Board of Biomass and Bioenergy, an international journal published by Elsevier (1/87 - 2005)

Member, Editorial Board of Computers and Electronics in Agriculture, an international journal published by Elsevier (1/84 - 12/90)

Awards And Honors:

2009 Elected as a Fellow of the American Institute of Medical and Biological Engineering
2009 Black Enterprise Magazine, Master of Innovation Award
2008 Outstanding Alumnus Award from the Michigan State University, College of Agriculture and Natural Resources
2007 Michigan State University Biological and Agricultural Engineering Distinguished Alumni Award
2006 Alice H. Cook and Constance E. Cook Award for support of women at Cornell
2006 New York Science, Technology and Academic Research (NYSTAR) Faculty Development Award
2005 Recognized for Outstanding Contributions as a member of the Biomass Research and Development Committee, the National Biomass Initiative, U.S. Dept. of Energy and U.S. Dept. of Agriculture
1997 Stephen '57 and Marilyn Miles College of Engineering Teaching Award
1996 The Howard University Outstanding Achievement Award
1994 Member, Gamma Sigma Delta - The Honor Society of Agriculture
1990 Faculty Advisor Award from the National Society of Black Engineers Cornell University
1985 Ford Foundation Postdoctoral Fellowship for Minorities.
1975 Member, Alpha Epsilon - Agricultural Engineering Honor Society

Patents:

Wilson, D. B., Walker, L. P. and Zhang, S. 1997. Thermostable cellulase from a thermomonospora gene. United State Patent No. 5,677,151.

Refereed Publications (1998 Thru Present):

Walker, L. P. 2009. Managing complexity and diversity in evolving bioenergy systems. ME Today, 11.

Munro, S. A., Zinder, S. H. and Walker, L. P. 2009. Fermentation stoichiometry of Thermotoga neapolitana and influence of temperature, oxygen, and pH on hydrogen production. Biotechnology Progress, In Press.

King, B., Donnelly, M., Bergstrom, G., Walker, L. P., Gibson. D. 2008 An optimized microplate assay system for quantitative evaluation of plant cell wall degrading enzyme activity of fungal culture extracts. Biotechnol. Bioengineering, 102: 1033-1043.

Moran-Mirabal, J. M., Santhanam, N., Corgie, S. C., Craighead, H. G., and Walker, L. P. 2008. Immobilization of Cellulose Fibrils on Solid Substrates for Cellulase Binding Studies Through Quantitative Fluorescence Microscopy. Biotechnol. Bioengineering, 101: 1129-1141.

Reccius,C. H., Stavis, S. M., Mannion, J. T., Walker, L. P. and Craighead, H. G. 2008. Conformation, length and speed measurements of electrodynamically stretched DNA in nanochannels. Biophysical Journal, 95: 273-286.

Santhanam, N. and Walker, L. P. 2008. A High-Throughput Assay to Measure Cellulase Binding and Synergism in Ternary Mixtures. Biological Engineering, 1: 1-19.

Pryor, S. W., Siebert, K. J., Gibson, D. M., Gossett, J. M. and Walker, L. P. 2007. Modeling Production of Antifungal Compound and their Role in Biocontrol Product Inhibitory Activity. J. Agric. Food Chem., 55: 9530–9536.

Krause, E. L., Villa-García, M. J., Henry, S. A. and Walker, L. P. 2007. Determining the Effects of Inositol Supplementation and the opi1 Mutation on Ethanol Tolerance of Saccharomyces cerevisiae. Industrial Biotechnol., 3: 260-268.

Pryor, S. W., Gibson, D. M., Hay, A. G., Gossett, J. M. and Walker, L. P. 2007. Optimization of Spore and Antifungal Lipopeptide Production during the Solid State Fermentation of Bacillus subtilis. Appl. Biochem. Biotechnol., 143: 63-79.

Stavis, S. M., Corgié, S. C., Cipriany, B. R., Craighead_, H. G. and Walker L. P. 2007. Single Molecule Analysis of Bacterial PCR Products in Submicrometer Fluidic Channels. Biomicrofluidics, 1: 1-13.

Yu, L-X, Gray, B. N., Rutzke,C. J., Walker, L. P., Wilson, D. and Hanson. M. 2007. Expression of Thermostable Microbial Cellulases in the Chloroplasts of Nicotine-Free Tobacco. J. Biotechnology, 131: 362-369.

Pryor, S. W., Gibson, D. M., Bergstrom, G. C., and Walker, L. P. 2007. Minimization of Between-well Sample Variance of Antifungal Activity Measurements Using a High-Throughput Screening Microplate Bioassay. BioTechniques, 42(2):151-156.

Pryor, S. W., Gibson, D. M., Krasnoff, S. B., and Walker, L. P. 2006. Identification of Antifungal Compounds in a Biological Control Product Using a Microplate Inhibition Bioassay. Trans. ASAE, 49: 1643-1649.

Jeoh, T., Wilson, D. B. and Walker, L. P. 2006. Effect of Cellulase Mole Fraction and Cellulose Recalcitrance on Synergism in Cellulose Hydrolysis and Binding. Biotechnology Progress, 22: 270-277.

Richards, T. L., Walker, L. P., and Gossett, J. M. 2006. Modeling the Temperature Kinetics of Aerobic Solid-State Biodegradation. Biotechnology Progress, 22: 70-77.

Richards, T. L., Walker, L. P., and Gossett, J. M. 2006. Effect of oxygen on aerobic solid-state biodegradation kinetics. Biotechnology Progress, 22: 60-69.

Kephart, K.D., Rutzke, C.J., Scott, N.R., Walker, L.P. 2005. The Sun Grant Initiative ? A New Day for Agriculture. Hort Sci. 40(2):Apr. 2005.

Hansgate, A. Schloss, P. D., Hay, A. G., and Walker, L. P. 2005. Molecular Characterization of Fungal Community Dynamics in the Initial Stages of Composting. FEMS Microbiology Ecology, 51: 209-214.

Schloss, P. D., Hay, A. G., Wilson, D. B., Gossett, J. M., and Walker, L. P. 2005. Quantifying Bacterial Population Dynamics in Compost using 16S rRNA gene probes. Applied Microbiology and Biotechnology, 66: 457-463.

Schloss, P. D., Hay, A. G., Wilson, D. B., and Walker, L. P. 2003. Tracking Temporal Changes of Bacterial Community Fingerprints During the Initial Stages of Composting. FEMS Microbiology Ecology, 46: 1-9.

Jung, H., Wilson, D. B. and Walker, L. P. 2003. Binding and reversibility of Thermobifida fusca Cel5A, Cel6B and Cel48A and their respective Catalytic Domains to Bacterial Microcrystalline Cellulose. Biotechnology and Bioengineering, 84: 151-159.

Howeler, M., Ghiorse, W. C., and Walker, L. P. 2003. A Quantitative Analysis of DNA Extraction and Purification from Compost. J. Microbiological Methods, 54: 37-45.

Schloss, P. D., Hay, A. G., Wilson, D. B., and Walker, L. P. 2002. Molecular Assessment of Inoculum Efficacy and Process Reproducibility in Composting using ARISA. Trans. ASAE, 46: 919-927.

Jung, H., Wilson, D. B. and Walker, L. P. 2002. Binding of Thermobifida fusca CDCel5A, CDCel6B and CDCel48A to easily hydrolysable and recalcitrant cellulose fractions on BMCC. Enzyme and Microbial Technology, 31: 941-948.

Jung, H., Wilson, D. B. and Walker, L. P. 2002. Binding mechanism for Thermobifida fusca Cel5A, Cel6B and Cel48A binding domains on bacterial microcrystalline cellulose. Biotechnology and Bioengineering, 80: 380-392.

Jeoh, T., Wilson, D. B. and Walker, L. P. 2002. Cooperative and Competitive Binding in Synergistic Mixtures of Thermobifida fusca Cellulases Cel5A, Cel6B, and Cel9A. Biotechnology Progress, 18: 760-769.

Pryor, S., Hay, A., and Walker, L. P. 2002. Nonylphenol in anaerobically digested sewage sludge from New York State. Environmental Science and Technology: 36, 931-942.

Watson, D. L. and Walker, L. P. 2002. Synergism in binary mixtures of Thermobifida fusca cellulases Cel6B, Cel9A and CEL5A on BMCC and Avicel: influence of cellulase mole fraction and concentration. Applied Biochemistry and Biotechnology, 101: 97-111

Schloss, P. D. and Walker, L. P. 2001. Assessment and improvement of process variable repoducibility in composting reactors. Trans. ASAE, 44: 1022-1030.

Heilig, A., DeBruyn, D., Walter, M. T., Rose, C. W., Parlange, J.-Y., Steenhuis, T. S., Sander, G. C., Hairsine, P. B., Hogarth, W. L., and Walker, L. P. 2001. Testing a mechanistic soil erosion model with a simple experiment. J. Hydrology, 224: 9-16.

Higgins, C. W. and Walker, L. P. 2001. Validation of a new model for aerobic organic solids decomposition: simulations with substrate specific kinetics. Process Biochemistry, 36: 875-884.

Schloss, P. D. and Walker, L. P. 2000. Measurement of process performance and variability in inoculated composting reactors using ANOVA and power analysis. Process Biochemistry, 35: 931-942.

Schloss, P. D., Chaves, B. and Walker, L. P. 2000. The use of the analysis of variance to assess the influence of mixing during composting. Process Biochemistry, 34: 601-612.

Walker, L. P., Nock, T. D., Gossett, J. M. and VanderGheynst, J. S. 1999. The Role of Periodic Agitation and Water Addition in Managing Moisture Limitations During High-Solids Aerobic Decomposition. Process Biochemistry, 34: 601-612.

Richard, T. L., Walker, L. P. and Gossett, J. M. 1999. The Effects of Oxygen on Solid-State Biodegradation Kinetics. Proceedings of the Inst. of Biological Engineering 2: A10-A30.

Baker, C. S., VanderGheynst, J. S. and Walker, L. P. 1999. Equilibrium Moisture Isotherms for Synthetic Food Waste and Biosolids Compost. Compost Science, 7: 6-13

VanderGheynst, J.S., Cogan D.J., DeFelice P.J., Gossett J.M. andWalker L.P. 1998. Effect of Process Management on the Emission of Organosulfur Compounds and Gaseous Antecedents from Composting Processes. Environ. Sci. and Technol., 32: 3713-3718.

Richard, T. L. and Walker, L. P. 1998. Temperature Kinetics of Aerobic Solid-State Biodegradation. Proceedings of the Inst. of Biological Engineering 1:A22-A39.

Wheeler, E. F., Albright, L. D., Spanswick, R. M., Walker, L. P. and Langhans, R. W. 1998. Nitrate Uptake kinetics in Lettuce as Influenced by Light and Nitrate Nutrition. Trans. ASAE, 41: 859-867.

Champenois, Y. F., Rao, A. M. and Walker, L. P. 1998. Influence of ?-amylase on the Viscoelastic Properties of Starch-Gluten Pastes and Gels. J. Sci. Food Agric., 78: 119-126.

Champenois, Y. F., Rao, A. M. and Walker, L. P. 1998. Influence of Gluten on the Viscoelastic Properties of Starch Pastes and Gels. J. Sci. Food Agric., 78: 127-133.

Kim, E., Irwin, D. C., Walker, L. P. and Wilson, D.B. 1998. Factorial Optimization of a six cellulase mixture. Biotechnol. Bioengnr., 58( 5): 494-501.

VanderGheynst, J. S., VanderGheynst, G. B. and Walker, L. P. 1997. Measuring oxygen in compost piles. BioCycle, 38 (10): 72&74.

VanderGheynst, J. S., Walker, L. P. and Parlange, J. -Y. 1997. Energy Transport in a High-Solids Aerobic Degradation Process: Mathematical Modeling, Validation and Analysis. Biotechnology Progress, 13 (3): 238-248.

Oppenheimer, J. R., Martin, A. G. and Walker, L. P. 1997. Measurement of air filled porosity in unsaturated organic matrices using a pycnometer. Bioresources Technology, 59: 241-247.

VanderGheynst, J. S., Gossett, J. M. and Walker, L. P. 1997. High-solids aerobic decomposition: pilot-scale reactor development and experimentation. Process Biochemistry, 32 (5): 361-375.

VanderGheynst, J. S., VanderGheynst, G. B. and Walker, L. P. 1997. Development and analysis of oxygen sensing probes for in-situ monitoring of solid-state biodegradation processes. Journal of the Air and Waste Management Association, 47: 642-651.

Bothwell, M. K., Wilson, D. B., Irwin, D. C. and Walker, L. P. 1997. Binding reversibility and surface exchange of Thermomonospora fusca E3 and E5, and Trichoderma reesei CBHI. Enzyme and Microbial Technol., 20: 411-417.

Bothwell, M. K., Daughetee, S. D., Chau, G. Y., Wilson, D. B. and Walker, L. P. 1997. Binding capacities of Thermomonospora fusca E3, E4 and E5, the E3 binding domain, and Trichoderma reesei CBHI on Avicel and bacterial microcrystalline cellulose. Bioresources Technology, 60: 169-178.

Articles:

Gaudioso, J., Miller, D.E., Turner, S.W.P., Jeoh, T., Walker, L.P., and Craighead, H.G. 2001. Diffusion Based Protein Separation in Microfluidic Devices. In: Micro Total Analysis Systems 2001, J.M. Ramsey and A. van den Berg (eds.), Kluwer Academic Publishers, Netherlands, pages 107-108.

Bothwell, M. K., Walker, L. P., Wilson, D. B., Irwin D. C. 1997 Binding Reversibility and Surface Exchange of Trichoderma reesei CBHI and Thermomonospora fusca E3 and E5 Cellulases. In Advances in Food Engineering, G. Narsimhan, M.R. Okos and S. Lombardo, (eds.), Purdue University, West Lafayette, IN, pages 6-9

Compiled Research Reports:

Walker, L. P. and J. M Gossett. 2000. Controlling Odors and Waste Stabilization in Composting System Through Process Design, Analysis, and Monitoring. Final Report, New York State Energy Research and Development Authority, Report 00-11, August 2000, 130 pp.

Walker, L. P. and D. B. Wilson. 1997. Optimizing Cellulase Mixtures for Maximum Rate and Extent of Hydrolysis. Final Report, New York State Energy Research and Development Authority, Report 97-6, March 1998, 60 pp.

Recent Invited Presentations:

Walker, L.P. 2009. Pursuing Industrial Biotechnology Approaches for Bioenergy Solutions, NanoFocus and Bioenergy, Oklahoma EPSCoR Annual State Conference, Oklahoma City, Mar. 31-Apr. 1.

Walker, L.P. 2009. Conference Chair, Welcome Address and Moderator, The Sun Grant Initiative Energy Conference, L’Enfant Plaza, Washington, DC, Mar. 10-13.

Walker, L.P. 2009. Biofuels and Industrial Biotechnology Development in NY State, Empire State Council of Agricultural Organizations, Syracuse, NY, Jan. 9.

Walker, L.P. 2008. Complexity and Exploiting Diversity in Evolving Bioenergy Systems Advanced Energy Conference “Solutions to a Global Crisis,” Hyatt Regency Windwatch, Hauppauge, NY, Nov. 19-20.

Walker, L.P. 2008. Biofuels and Industrial Biotechnology Development in NY State, NY Association of Conservation Districts (NYACD) Annual Meeting, Geneva, NY, Oct. 19-21.

Walker, L.P. 2008. Participant in Joint Workshop, US Dept. of Energy/Office of Science and the US Dept. of Agriculture joint workshop on “Sustainabillity of Biofuels: State of the Science and Future Directions,” Bethesda Marriott Hotel, Bethesda, Maryland, October 28-29.

Walker, L.P. 2008. Focusing the Lens of the Life Sciences through Nanobiotechnology on Bioenergy Solutions, Trustee-Council Annual Meeting, Cornell University Campus, Ithaca, NY, October 18.

Walker, L.P. 2008. Invited Participant on the Planning Committee, USDA Energy Research, Education, and Extension Strategic Energy Planning Summit, Marriott Mayflower Hotel, Washington, DC, Sept. 29-30.

Walker, L.P. 2008. Exploring Nanotechnology and Industrial Biotechnology Nexuses, 2008 Pacific Rim Summit on Industrial Biotechnology and Bioenergy, Vancouver, BC, Canada, Sept. 10-12.

Walker, L.P. 2008. Welcome Address, and Leading Breakout Session entitled, Biomass 102, Northeast Renewable Energy Conference, Penn State University, State College, PA, Aug.25-28.

Walker, L.P. 2008. Invited Keynote Speaker, American Chemical Society 236^th National Meeting, AGRO Division, Philadelphia, PA, Aug. 17-21.

Walker, L.P. 2008. Invited Presentation to the Renewable Energy Task Force, Subcommittee on Renewable Energy, on the First Report to Gov. David A Patterson: Clean, Secure Energy and Economic Growth: A Commitment to Renewable Energy and Enhanced Energy Independence, SUNY ESF, Syracuse, NY.

Walker, L.P. 2008. Complexity and Exploiting Diversity in Evolving Bioenergy Systems. Invited Speaker, Advanced Energy Conference, “Solutions to a Global Crisis.” SUNY Stonybrook, Islip, NY.

Walker, L.P. 2008. From Field to Fuel: Growing Biofuels in New York State. Environmental Business Association of New York State, Biofuels Industries Conference, Saratoga Springs, NY.

Walker, L.P. 2008. Industrial Ecology. Invited presentation at USDA Monthly Seminar Series with Undersecretary Gale Buchanan, Washington, DC.

Walker, L.P. 2008. The Evolving Paradigm of Agriculture as a Supplier of Energy and Industrial Chemicals. Invited Presentation at the Cornell/Tsinghua University Joint Workshop, Cornell University, Ithaca, NY.

Walker, L.P. 2008. Transitioning to a Sustainable Global Energy Future. Invited presentation at The Parents Campus Visit Program, Cornell Campus, Ithaca, NY.

Walker, L.P. 2008. Catalyzing the Development of a Domestic Biofuels Sector. Invited presentation to the Cornell Engineering Alumni Association (CEAA) Annual Conference, “Sustainable Energy Systems: Investing in our Future,” Cornell University, Ithaca, NY.

Walker, L.P. 2008. The Evolving Paradigm of Agriculture as a Supplier of Energy and Industrial Chemicals. Invited presentation at the 2008 Empire State Fruit and Vegetable Expo: Alternative Energy Session, Syracuse, NY.

Walker, l..P. 2008. Imagine your Energy Future. Invited speaker for program at Boynton Middle School, Ithaca, NY, Black History Month: What Does it Mean to Me?

Walker, L.P. 2008. University/Industry Partnerships Panel. Invited panel member at the Upstate Sustainability Symposium: Focus on Energy. Pittsford, NY.

Walker, L.P. 2008. Research and Market Evolution - opportunities for NY in the Alternative Energy Arena. Invited speaker at the NYS Agricultural Society Annual Meeting and Agricultural Forum. Liverpool, NY.

Walker, L.P. 2008. Exploring Nanotechnology and Industrial Biotechnology Nexuses. Session Organizer and Moderator. 2008 Pacific Rim Summit on Industrial Biotechnology and bioenergy, Vancouver, BC, Canada.

Walker, L.P. 2008. Overview of Cornell Biofuel Activities. Invited presentation and conduct tour of the Biofuels Research Laboratory at Cornell with representatives from Lockheed Martin, Energy Initiative and Deployable Fuel Systems Development Sections.

Walker, L.P. 2008. Focusing the Lens of the Life Sciences through Nanobiotechnology on Bioenergy Solutions. Invited presentation at the Trustee-Council Annual Meeting, Cornell University, Ithaca, NY.

Walker, L.P. 2008. Energy Challenges and Conservation. Invited roundtable member at the New York Association of Conservation Districts Annual meeting, Geneva, NY.

Walker, L.P. 2008. The Evolving Paradigm of Agriculture as a Supplier of Energy and Industrial Chemicals. Invited Keynote Presentation, American Chemical Society 236^th National Meeting, Philadelphia, PA.

Walker, L.P. 2008. Invited Participant, USDA Energy Research, Education and Extension Strategic Energy Summit. Washington, DC.

Walker, L.P. 2008. Northeast Renewable Energy Conference, Breakout Sessions Moderator, “Biochemical Fuel Conversions,” and “NESGI, Revisiting the Roadmap for the Northeast.” Penn State University, State College, PA.

Walker, L.P. 2008. Invited participant in the joint workshop of the U.S. Dept. of Energy/Office of Science and the U.S. Dept. of Agriculture, “Sustainability of Biofuels: State of the Science and Future Directions.” Bethesda, MD.