 | John March, Ph.D. Assistant Professor Profile and CV | 220 Riley Robb jcm224@cornell.edu Web site |
| Signal transduction engineering, synthetic biology, bacterial gene therapy, metabolic engineering | ||
Biography
John March joined the faculty in 2005 after completing his Ph.D. in Chemical Engineering at the University of Maryland, College Park, MD. Before that he earned an M.S. in Biological Engineering at the University of Georgia in Athens, GA. He worked for several years as a research engineer prior to completing his M.S.
Research
The current research in the March lab is focused on metabolic signal transduction. Cellular populations respond to their environments through almost instantaneous signaling cascades, which can completely change cellular metabolism and physiology. The March lab is working to understand cellular metabolism and signaling and to use these systems to develop novel technologies. Please see the Metabolic and Signal Engineering Lab homepage for more information.
Professional CV
Education:
- Ph. D. Chemical Engineering, University of Maryland, College Park, May 2005.
- M.S. Biological Engineering, University of Georgia, August 2000.
- B.S. Biological Engineering, University of Georgia, May 1996.
Professional Work Experience
Center for Biosystems Research, University of Maryland Biotechnology Institute: 2000-2005.
Graduate Research Assistant in the area of eukaryotic heterologous protein synthesis.
- Developing a novel approach to enhance gene expression in eukaryotic model systems through post-transcriptional gene silencing (RNAi).
- Investigating molecular biology of eukaryotic signal transduction.
Center for Molecular Bioengineering, University of Georgia: 1998-2000
M.S. Candidate under a departmental assistantship in Biological Engineering:
- Developed metabolic engineering approaches to increase yield of heterologous protein.
- Modified central metabolism of E. coli for increased flux to anaplerotic intermediates.
American Technologies, Incorporated, Aiken, SC: 1996-2000
Project Biological Engineer:
- Served as project engineer and site manager for a research Aerobic Landfill Bioreactor (ALB).
- Provided data preparation, interpretation, and modeling services.
- One of four inventors for the ALB patents.
Enzyme Engineering, University of Georgia: 1993-1996
Served as an investigator for state funded fire ant and environmental research:
- Developed a novel hypothesis for fire ant control.
- Designed experiments and wrote software modeling phytoremediation of heavy metals.
Professional Societies:
- ACS
- ASM
- IBE
- AICHE
- SBE
- AAAS
Patents and Publications:
March, J. C. and Bentley, W. E. (2006) "Signal transduction interference for improved product yield in recombinant eukaryotic cell culture." Applied Microbiology and Biotechnology; [Epub ahead of print]
March, J. C. and Bentley, W. E. (2006) "Engineering eukaryotic signal transduction with RNAi: enhancing Drosophila S2 cell growth via TSC1." Biotechnology and Bioengineering, 95: 645-652.
Wang, L., Li, J., March, J. C., Valdes, J. J. and Bentley, W. E. (2005) "luxS-Dependent gene regulation in Escherichia coli K-12 revealed by genomic expression profiling." Journal of Bacteriology, 187: 8350-8360.
March, J. C. and Bentley, W. E. (2004) " Quorum sensing and bacterial cross-talk in biotechnology ." Current Opinion in Biotechnology, 15: 495-502.
March, J. C. and Bentley, W. E. (2004) "Insulin stimulates uptake of dsRNA in Drosophila S2 cells." Biotechniques, 36: 898-900.
March, J. C., Rao, G., and Bentley, W. E. (2003) "Biotechnological applications of the green fluorescent protein." Applied Microbiology and Biotechnology, 62: 303-315.
March, J. C., Altman, E., and Eiteman, M. A. (2002) "Expression of anaplerotic enyzme pyruvate carboxylase improves recombinant protein production in Escherichia coli." Applied and Environmental Microbiology, 68: 5620-5624.
Hudgins, M. A., Bessette, B., March, J. C., and McComb, S. (2000 and 2002) Aerobic Landfill Bioreactor, U.S. Patents 6,024,513 and 6,364,572.
Hudgins, M. A., Bessette, B., March, J. C., and McComb, S. (2000 and 2002) Aerobic Landfill Bioreactor, U.S. Patents 6,024,513 and 6,364,572.
Posters and Presentations (presentations at the local level not shown):
March, J. C. and Bentley, W. E. (2003) "Post-transcriptional gene silencing in the metabolic engineering of Drosphila melanogaster ". Poster at the AIChE National Meeting, San Fransisco, CA.
March, J., Eiteman, M. A., Schwaner, R., and Altman, E. (2000) "Overexpression of pyruvate carboxylase in E. coli increases production of recombinant b-galactosidase." Oral presentation to ACS National Meeting, San Francisco, CA.
March, J., Eiteman, M. A., and Altman, E. (1999) "Increasing recombinant protein yield in E. coli fermentations." Oral presentation to GA section of ASAE Fall Meeting, Athens, GA.
March, J., Schwaner, R., Eiteman, M. A., and Altman, E. (1999) "Metabolic engineering of E. coli for increased flux to biosynthetic precursors." Poster at the Recent Advances in Fermentation Technology (RAFT) III Conference, Sarasota, FL.
Honors and Activities:
Session Chair, Mid-Atlantic Bioengineering Conference (MABEC), UMD, 2003.
President, Chemical Engineering Graduate Students (ChEGS), UMD, 2001-2002.
Treasurer, ChEGS, 2000-2001.
Member of American Chemical Society (ACS), BIOT division for 7 years.
Representative, CAES Graduate Council, 1998-2000.
Representative, BAE Graduate Advisory Committee, 1998-2000.
Recipient, BAE Graduate Enhancement Award, 1999.
Nominated for E. Brodus Browne Award, 1999.
President, Graduate Engineering Club, 1998-1999.
Founding Member of Institute of Biological Engineering, 1995.
Secretary and founding member, Biological Engineering Club at UGA, 1995-1996.
Scholarship Recipient, GA Section of ASAE Scholarship, 1995-1996.
Scholarship Recipient, Jean Dewitt Fitz Memorial Scholarship, 1991.
Co-editor, Engineering at UGA, 1995-1996.
Editor, Stillpoint Magazine, 1990 and 1991.